Japan Bioinformatics

Simbiot provides a convenient method for quantifying expression levels for genes and transcripts.  Quantification is done using cufflinks, which produces values normalized by transcript length and htseq-count, which calculates un-normalized expression values.  In general, normalized expression measures are better used for analyzing isoforms within samples and un-normalized values for differential expression between samples.  The user can easily apply variance reduction algorithms as well as transformation and normalization to the data matrixes and conduct down-stream expression analysis experiments